- Bloomington Drosophila Stock Center
- Drosophila Genomics Resource Center
- Caenorhabditis Genetics Center
- Center for C. elegans Anatomy
- WormGuides: a resource for Global Understanding in Dynamic Embryonic Systems
- National Resource for Aplysia
- Resource Center for Tetrahymena Thermophila
The center collects, maintains and distributes genetically defined strains of Drosophila melanogaster with significant research value. Emphasis is placed on strains providing experimental tools useful to a broad range of investigations including marker, balancer, mapping, and transposon-tagging strains; mutant alleles of identified genes including a large set of transposable element insertion alleles; defined sets of chromosomal deficiencies and duplications; engineered lines for somatic and germline clonal analysis; RNAi lines for targeted gene knockdown; GAL4, UAS, QF and QUAS lines for targeted gene expression; enhancer trap and reporter strains with defined expression patterns for marking tissues; transposon-induced lethal mutations; engineered lines for high efficiency transgenesis; fully sequenced wild type strains; and lines for modeling human diseases and health-related processes.
Approximately 41,000 fly strains are currently in distribution. Up-to-date stock lists are available for searching, browsing, or downloading/copying at the Internet site .
New users must contact the center for a Bloomington user number (BUN) before ordering via a web-based automated ordering system. One small starter culture is provided of each requested stock; larger cultures cannot be provided. Visit the website for information on ordering stocks and current fees.
Stock center scientists are available to answer questions about strains, use of the website and database files, and Drosophila genetics for researchers new to the field.
Bloomington Drosophila Stock Center
Department of Biology
1001 East 3rd Street
Bloomington, IN 47405-3700
Kevin R. Cook, Ph.D.
Contact: Annette L. Parks, Ph.D.
Kathleen A. Matthews, Ph.D.
Thomas C. Kaufman, Ph.D.
The Drosophila Genomics Resource Center (DGRC) collects and distributes reagents and materials essential for Drosophila genomics research, including large clone sets, common transformation vectors, and cell lines. It also tests and refines emerging genomics technologies for Drosophila, supports work on Drosophila cell lines, and advises users in the use of resources.
Clones: A collection of over 1,000,000 clones is distributed as individual clones and sets. For each clone in the collection, the DGRC website provides relevant references, sequence information, restriction maps, and links to FlyBase entries
Transformation Vectors: The Center distributes general-purpose transformation vectors for use in flies or in cultured cells.
Cell lines: The DGRC distributes over 100 Drosophila cell lines, provides website support for their use and handling, characterizes newly-added lines, and collaborates with other on-going projects (modENODE, the Drosophila RNAi Screening Resource) to enhance and disseminate systems level information on the cell lines.
Microarray Resources: The facilitates users’ use of Drosophila transcriptome microarrays through the Center for Genomics and Bioinformatics at Indiana University, Bloomington. DGRC bioinformaticists are developing tools facilitate the analysis of these data.
Users must create an account prior to purchasing. In addition to purchase orders, online credit card orders are alo accepted. Fees are adjusted on an annual basis.
The Center for Genomics and Bioinformatics
Jordan Hall A311
1001 East 3rd Street
Bloomington, IN 47405-3700
Website: http://dgrc.cgb.indiana.edu/Principal Investigator
Peter Cherbas, Ph.D.
812-855-6273; Fax: 812-856-9340
Claire Selzer, Ph.D.
The Caenorhabditis Genetics Center (CGC) acquires, maintains, and distributes genetic stocks and information about stocks of the small free-living nematode Caenorhabditis elegans for use by investigators initiating or continuing research on this genetic model organism. The CGC maintains a searchable strain database accessible from the CGC website . This site also provides general information about C. elegans and links to key websites of use to scientists, including WormBase .
The CGC's collection of more than 17,000 strains includes one allele of each mapped gene, all available chromosome rearrangements, and selected multiple-mutant stocks for genetic mapping. Certain transgenic strains also are available, including strains that express various green fluorescent protein (GFP) reporter fusions. The CGC also has stocks of nematode species closely related to C. elegans and bacterial strains necessary for nematode growth and for performing RNA interference experiments. Information about CGC stocks can be obtained from the CGC website.
Caenorhabditis Genetics Center
Department of Genetics and Cell Biology
University of Minnesota
6-160 Jackson Hall
321 Church St., SE
Minneapolis, MN 55455
Ann E. Rougvie, Ph.D.
612-625-2265; Fax: 612-625-4648
The Center does ultrastructural research on the anatomy and development of the model organism, Caenorhabditis elegans. Transmission and scanning electron microscopic (TEM and SEM) studies are conducted in collaboration with established C. elegans researchers and with new students and postdoctoral fellows. Tissue defects in selected mutant alleles are compared with normal nematode tissues.
Electron microscopic (EM) techniques are being developed and improved for C. elegans studies, including better fixations and embeddings for immunoEM and histochemical studies. Special emphasis is being placed on electron tomography methods for viewing cell organelles in three dimensions, and on SEM preparation methods (FIB/SEM, ATUM) for whole animal reconstruction from serial sections.
An archive of TEM images has been assembled for the normal animal—adults, larvae, embryos, and in both sexes. The data are being used to create an online database (WORMATLAS ) featuring a Handbook of Anatomy, a Glossary, and the Slideable Worm, where all tissues are displayed in relation to the whole body. Our online Community Forum answers queries regarding EM methods and specific details of nematode anatomy. An online searchable image database (WORMIMAGE ) presents thousands of original micrographs from the TEM Archive. Our goal is to make the information free to all researchers and teachers interested in nematode anatomy and development.
Several major themes are being followed. These include the ultrastructural anatomy of aging, axon guidance, cell death and autophagy, intercellular fusion, and gonadal development. Synaptic wiring in wild type and mutant animals is studied in serial thin sections; a principal effort is to complete the neuronal wiring of the adult male nervous system.
High-pressure freezing and microwave protocols are being compared as methods for immunocytochemistry, electron tomography and for mutant analysis.
Advanced training is available on a one-to-one basis to learn EM methods for C. elegans; we prefer that the student have some background in electron microscopy in advance. Techniques include fixation, embedding, serial thin sections, antibody staining, and microscopy.
Our technical staff can help in conducting research projects using these methods. The work is often shared with the collaborating laboratory, so that an outside investigator learns to use the microscope, collect images, and analyze the data.
Select portions of the IMAGE Archive are available upon request by FTP or on DVDs.
Center for C. elegans Anatomy
1300 Morris Park Avenue
Kennedy Center, Room 601
Albert Einstein College of Medicine
Bronx, New York 10461
David H. Hall, Ph.D.
718-430-2195; Fax: 718- 430-8821
Zeynep F. Altun, M.D., Ph.D.
Laura A. Herndon, Ph.D.
The WormGUIDES consortium is engaged in the construction of a 4D interactive database of dynamic behaviors of every cell throughout C. elegans embryogenesis at subcellular and minute-level resolution. The goal is to support examination and analysis of complex tissues at the molecular, cellular, tissue and organismal levels, to enable collaborative efforts of such analyses, and to facilitate sharing of such results.
WormGUIDES consists of computationally constructed models of cells and the embryo, the underlying time-lapse images, as well as Graphical User Interfaces and other software tools to enable analysis and sharing.
The current goal of WormGUIDES is to track the nuclear position of every cell (~1340) and neurite growth of 20 selected neurons. Longer term goals include neurite growth for the entire nervous system, as well as cell fate differentiation and cell shape for other cells by integrating information from other public sources such as the community databases of WormBase and WormAtlas, and contributions from the community.
The technologies developed in association with WormGUIDES, which are open-source and freely available, can be readily used to study complex tissues in other organisms. These include novel microscopy, image analysis software to track cells/cell lineage and trace cell shapes, as well as database schemes to integrate complex information and sharing of Big Data.
Keywords: Database, software tools, molecular markers in the forms of C. elegans strains and constructs.
Principal Investigators (In alphabetical order)
Zhirong Bao, PhD
Developmental Biology Program
Memorial Sloan Kettering Cancer Center
1275 York Ave, Box 416
New York, NY 10065
Daniel A. Colón-Ramos, PhD
Department of Cell Biology
Yale Program in Cellular Neuroscience, Neurodegeneration and Repair
Yale School of Medicine
295 Congress Avenue, BCMM 436B
New Haven, CT 06510
William A. Mohler, PhD
Dept. Of Genetics and Developmental Biology
UConn Health Center 400
Farmington, CT. 06030-6403
Hari Shroff, PhD*
National Institute of Biomedical Imaging and Bioengineering
13 South Drive, Room G800
Bethesda, MD 20892
(* Hari Shroff is a strategic collaborator whose research is not funded by the grant)
The National Resource for Aplysia (http://aplysia.miami.edu/ ) provides investigators with laboratory-reared Aplysia californica of known age and standardized environmental background at all stages of development from egg to mature adults, as well as red algae to feed animals.
The primary goal of the resource is to optimize and standardize Aplysia used by NIH investigators. This includes a health monitoring program and studies to optimize larval rearing and diet at all life stages. Current research programs focus on investigating changes associated with aging and onset of senescence. Landmarks of aging will be used to produce quantifiably aged animals for researchers.
Sibling animals of known ages and stages are available to investigators throughout the year. On request, Aplysia and their food (red algae) are shipped via Federal Express, overnight priority. Special cohorts, procedures, or manipulations of animal groups can be arranged by contacting the Resource.
The colony currently contains more than 10,000 laboratory-reared animals at various life stages. All animals are produced from field-collected brood stock or field-collected animals bred to lab reared brood stock, monitored for health, and randomly tested for behavioral responses prior to shipping.
Facility staff will provide advice on setting up marine aquarium facilities for short term holding of Aplysia shipped from the resource.
Guest Investigators and Graduate Students
Guest investigators and graduate students interested in studies of life history, culture, genetics, and neurophysiology are encouraged to inquire. Selection will be based on the relevance of the proposed study and the availability of resources to meet individual needs.
National Resource for Aplysia
Rosenstiel School of Marine and Atmospheric Science
University of Miami
4600 Rickenbacker Causeway
Miami, FL 33149
Michael C. Schmale, Ph.D.
This resource provides a centralized repository for genetically distinct strains of Tetrahymena thermophila, a ciliated protozoan that has served as a key model for studies of eukaryotic cellular and molecular biology for more than 50 years. The stock center will: 1) collect, annotate, and store experimentally useful cell lines; 2) establish a database that will provide essential information about these strains to the community at-large; and 3) supply actively growing cultures to researchers around the world.
Resource Center for Tetrahymena Themophila
Department of Microbiology and Immunology
College of Veterinary Medicine
Ithaca, NY 14853
607-253-4042; Fax: 607-253-3384
This page last reviewed on October 28, 2015